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Enzyme-linked Immunosorbent Assay (ELISA)
SPAR
kr 134
Enzyme-linked Immunosorbent Assay (ELISA)
This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.

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This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.

Produktdetaljer
Sprog: Engelsk
Sider: 115
ISBN-13: 9789811067655
Indbinding: Paperback
Udgave:
ISBN-10: 9811067651
Kategori: Immunologi
Udg. Dato: 22 jan 2018
Længde: 10mm
Bredde: 158mm
Højde: 235mm
Oplagsdato: 22 jan 2018
Forfatter(e) Patricia Vazquez-Villegas, Samira Hosseini, Sergio O. Martinez-Chapa, Marco Rito-Palomares


Kategori Immunologi


Sprog Engelsk


Indbinding Paperback


Sider 115


Udgave


Længde 10mm


Bredde 158mm


Højde 235mm


Udg. Dato 22 jan 2018


Oplagsdato 22 jan 2018

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